The Effeciency of Cryotop in Vitrification of In Vitro Produced Egyptian Buffalo (Bubalus Bubalis) Embryos
نویسندگان
چکیده
منابع مشابه
IN VITRO PRODUCTION OF BUFFALO (Bubalus bubalis) EMBRYOS
Two experiments (Exp) were carried out to test the effect of different media on in vitro maturation (IVM) (Exp 1; n = 1142 ovaries with 2666 oocytes) and to study in vitro fertilization (IVF) of buffalo oocytes (Exp 2; n = 110 ovaries with 330 oocytes). In exp 1 the collected cumulus oocyte complex (COCs) was allocated to three maturational media; TCM-199 supplemented with hormones (M1), TCM-19...
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Assisted reproductive technologies such as artificial insemination (AI) and multiple ovulation and embryo transfer (MOET) have been introduced to overcome reproductive inefficiencies and accelerate genetic gain. However, MOET in water buffalo is compromised by the poor ovulatory response of this animal species to hormonal treatment (Karaivanov 1986; Karaivanov et al. 1990; Misra et al. 1990; Cr...
متن کاملImprovement of vitrification of in vitro produced buffalo embryos with special reference to sex ratio following vitrification
Cryopreservation and sexing of embryos are integrated into commercial embryo transfer technologies. To improve the effectiveness of vitrification of in vitro produced buffalo embryos, two experiments were conducted. The first evaluated the effect of exposure time (2 and 3 min) and developmental stage (morula and blastocysts) on the viability and development of vitrified buffalo embryos. Morphol...
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Following fertilization, the early embryo is transcriptionally silent and early development is directed by the complement of maternally-inherited mRNAs and proteins. At some point, however, a maternal to embryonic transition occurs, in which further development is directed by the zygotic transcripts. So to determine this transition stage, the quantitative expression pattern of HMGN-2 and CREB g...
متن کاملThe in vitro effect of leptin on semen quality of water buffalo (Bubalus bubalis) bulls
The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...
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ژورنال
عنوان ژورنال: Journal of Animal and Poultry Production
سال: 2017
ISSN: 2090-3723
DOI: 10.21608/jappmu.2017.45784